Scil Proteins Services purifies proteins from various sources, from
E. coli but also from yeast or mammalian cells. Cell disruption, protein refolding, capture, purification, and polishing steps are carried out, optimised and, if necessary, documented with an accuracy that is mandatory for regulatory authorities.
The development of efficient and cost-effective purification protocols within reasonable time-limits requires both well experienced personnel and state-of-the-art industrial instrumentation.
Dependent on the inherent protein properties, Scil Proteins Services chooses the appropriate purification methods/principles to remove all undesired contaminants such as host cell protein (HCP) and pyrogenes/endotoxins and DNA. Precipitation and filtration methods, affinity, ionic exchange, hydrophobic interaction, hydroxyl apatite and size-exclusion chromatography, etc. are applied.
Protein purification should not be a matter of hope. It must be the result of an intelligent purification scheme where each single step has to be monitored by sensitive
physicochemical and biochemical analysis methods.
